Service information
Scantox offers the in vitro 3T3 neutral red uptake (NRU) phototoxicity test to enable the assessment of test substance phototoxicity to human skin under OECD 432: Phototoxicity Test. Certain chemicals only develop cytotoxic properties following activation by exposure to light. The 3T3 NRU phototoxicity assay provides a quick and high-throughput assessment of cytotoxicity induced only after test substance exposure to UV light, compared to non-light exposed conditions.
OECD 432 phototoxicity assay has been validated, and laboratory proficiency has been established at Scantox. Services are available in GLP and non-GLP formats.
| OECD guideline | OECD 432 |
| What is being assessed | Photoactivation of chemical cytotoxicity |
| Why is this important | Chemical exposure to humans and the environment inevitably leads to the subsequent exposure to UV, especially for substance with topical exposure. Determining whether a chemical is photoactivated or not, is a key phase in understanding risk assessment and management. |
| Test System | Balb/c 3T3 mouse fibroblast |
| Test Item Requirements | 200 mg |