Using a microwell format, the GLP Ames test, specifically the Ames 24-well Agar test, provides predictivity of the OECD 471 Ames study in comparable conditions, using just 5% of the test item. It is also conducted in solid agar. This makes the 24-well version of the Ames test highly amenable to screening, with TA98 & TA100 providing coverage for most known mutagenic substances.
Advantages for Screening
- Agar based, TA98 & TA100 ± S9
- Results comparable to full plate format
- Requires 20 mg compound
- Quick, cost-effective, high-quality results
| 24-well Test strains | TA98, TA100 |
| Metabolic activation | Typically, induced rat liver S9, other sources available |
| Replicates | 3 Wells per test concentration |
| Typical test item requirements | 20mg |
| Study Duration | < 3 Weeks |
| Endpoint | Mutagenicity |