A miniaturised version of a TK6 in vitro micronucleus test in which 2 treatment conditions are utilised in a 96-well microplate format. Micronucleus formation is assessed in 2000 mononuclear cells by microscopy, producing a highly predictive screen of the regulatory OECD 487 endpoint from minimal test item. The study can also be designed to incorporate FISH pan-centromeric staining techniques to enable additional assessment of micronuclei for the presence/absence of centromeres, predicting whether a material is predominately aneugenic or clastogenic.
Scantox also offers a version of the 96-well MNT test with automated scoring for faster data turnaround.
Advantages for Screening
- TK6 cell line
- Low compound requirement
- Prediction of OECD 487
- Incorporation of FISH gives prediction of mechanism
| MNT 96-well - Test Cell Line | TK6 |
| Metabolic activation | Typically, induced rat liver S9, though other sources can also be used Short +S9 Long –S9 2 arm study |
| Typical test item requirements | 20 mg (ICH S2(R1)) – < 100 mg (OECD 487) |
| + FISH | < 30 mg |
| Study Duration | < 4 Weeks (TK6 MNT) < 6 Weeks (TK6 MNT + FISH) |
| Endpoint | Micronucleus Formation / Mode of Action |