Service information
The OECD 487: In vitro MNT Test is a mainstay of most regulatory genotoxicity testing strategies and is used to detect in vitro clastogens and aneugens. In this regard, it complements the Ames test well, as it detects all of the three major classes of genotoxin between the two study types. Fluorescence In Situ Hybridisation (FISH) can also be conducted to differentiate predominantly clastogenic and aneugenic modes of action.
Scantox has conducted GLP in vitro MNT studies since 2014 in isolated human lymphocytes and TK6 cells. Our highly experienced team of scientists has been running, scoring, and study-directing this assay type for many years.
| Test systems | TK6 cells, or isolated human lymphocytes |
| OECD guideline | 487 |
| Metabolic activation | Typically induced rat liver S9, though other sources are available |
| Fluorescence In Situ Hybridisation (FISH) inclusion | Available in TK6 cells |
| Test Format | 3 hr +/- S9, and extended exposure -S9 Fluorescence microscopy pan-centromeric FISH probes, enable centromeric staining and analysis. |
| Typical test item requirements | 200 - 500mg |
| Formulation Analysis | Available upon request |
| Endpoint | Numerical and structural chromosomal damage |