Using multiple biomarkers within a single flow cytometry-based experiment, the MultiFlow® assay can distinguish between clastogenic and aneugenic modes of action and identify potentially misleading positives that might be secondary to cytotoxicity. This assay has considerable utility as a follow-up to positive in vitro results or as a primary screening tool, requiring low amounts of compound to generate fast predictions of mechanism and mode of action.
Advantages for Screening
- High throughput
- Low compound mode of action prediction
- Enables screening for aneugens, clastogens and non-genotoxic substance
| Test Cell Line | TK6 |
| Study Design | Typically, induced rat liver S9, other sources available. Sampling at 4hr & 24hr time points Biomarkers: p53, H2AX, H3, Polyploidy |
| Typical test item requirements | <20 mg |
| Study Duration | < 3 Weeks |
| Endpoint | Multiple biomarkers predict genotoxicity |